IJMS, Free Full-Text
Por um escritor misterioso
Last updated 21 setembro 2024
A template-assisted assembly approach to a C24 fullerene-like double-stranded DNA polyhedral shell is proposed. The assembly employed a supramolecular oligonucleotide dendrimer as a 3D template that was obtained via the hybridization of siRNA strands and a single-stranded DNA oligonucleotide joined to three- or four-way branched junctions. A four-way branched oligonucleotide building block (a starlet) was designed for the assembly of the shell composed of three identical self-complementary DNA single strands and a single RNA strand for hybridization to the DNA oligonucleotides of the template. To prevent premature auto-hybridization of the self-complementary oligonucleotides in the starlet, a photolabile protecting group was introduced via the N3-substituted thymidine phosphoramidite. Cleavable linkers such as a disulfide linkage, RNase A sensitive triribonucleotides, and di- and trideoxynucleotides were incorporated into the starlet and template at specific points to guide the post-assembly disconnection of the shell from the template, and enzymatic disassembly of the template and the shell in biological media. At the same time, siRNA strands were modified with 2′-OMe ribonucleotides and phosphorothioate groups in certain positions to stabilize toward enzymatic digestion. We report herein a solid-phase synthesis of branched oligodeoxy and oligoribonucleotide building blocks for the DNA/RNA dendritic template and the branched DNA starlet for a template-assisted construction of a C24 fullerene-like DNA shell after initial molecular modeling, followed by the assembly of the shell around the DNA-coated RNA dendritic template, and visualization of the resulting nanostructure by transmission electron microscopy.
IJMS, Free Full-Text
IJMS, Free Full-Text
IJMS, Free Full-Text
Plant Abiotic Stress
IJMS, Free Full-Text
Ijms Free Full Text Delayed Onset Of Age Dependent Changes In
IJMS, Free Full-Text
Ijms Free Full Text Preliminary Rna Seq Analysis Of Long Non
IJMS, Free Full-Text
Ijms Free Full Text Neural And Hormonal Basis Of 49680
Recomendado para você
-
The Mighty Thor #1 CGC 9.8 Hip Hop MF Doom Variant Jane Foster Love And Thunder21 setembro 2024
-
Hail Crow King-Of-Hell | Lloyd Bailey MF Doom Homage Foil Variant | CGC SS 9.8 | Comic Books - Modern Age, Crow21 setembro 2024
-
Amazing Spider-Man #54.LR CGC 9.821 setembro 2024
-
👑 Hail Crow: King of Hell #1 MF DOOM Homage. Javan Jordan. CGC21 setembro 2024
-
Something Blue Not Paper Towel21 setembro 2024
-
File:FRIPISA embalagem.jpg - Wikimedia Commons21 setembro 2024
-
Got some graded comics in. Hip hop in da Cave. Of course we have21 setembro 2024
-
Buy Top Tunes for Flute Online at $8.99 - Flute World21 setembro 2024
-
Thiago Barros on X: / X21 setembro 2024
-
Fusor 181 181 Double Sided Tape, 60 ft x 1/2 in x 0.045 in21 setembro 2024
você pode gostar
-
Get DRAGON BALL Z: KAKAROT Demo Version - Microsoft Store en-SA21 setembro 2024
-
SeaOfPixels on X: @JasperBoerstra @MukiTanuki Lodestones should21 setembro 2024
-
Como assistir tênis ao vivo US Open 2023 em qualquer lugar21 setembro 2024
-
How to Install GORILLA TAG Mods (BROKEN!)21 setembro 2024
-
Is The NEW Blizzard Fruit Really Worth Buying? (Roblox Bloxfruit)21 setembro 2024
-
Wolf Play - Online Wolf Game!21 setembro 2024
-
Baixar Rebaixados Elite Brasil 3.9 Android - Download APK Grátis21 setembro 2024
-
Shiny Moltres (My Version) by Lasercraft32 on DeviantArt21 setembro 2024
-
Avengers: Endgame 1:10 Star Lord Iron Studios BDS Art Scale Statue21 setembro 2024
-
Boneco Bebê - Reborn - Laura Baby - Mini Lino - Shiny Toys21 setembro 2024